This review focuses on current research achievements of nanomaterial‐assisted electrochemical sensors for food safety analysis reported in recent 5 years. With the assistance of nanomaterials, electrochemical sensors are a potential alternative to conventional methods of food safety analysis. Given their unique physical, chemical, and biological characteristics, nanomaterials are considered perfect candidates for the design of electrode surface to construct electrochemical sensors with excellent analytical performance. At present, nanomaterials are at the forefront of various electrochemical sensing applications. Thus, great emphasis is placed on sensitive, selective, and convenient analytical methods of various food safety hazards in food. Food safety risks caused by the existence of food safety hazards in food may occur in all stages of the food supply chain. Furthermore, detection of AFB1 in maize flour samples yielded recovery of 97–99%, in a demonstration of the possible use of the paper-based immunosensor to detect AFB1 using extraction solutions from food samples.įood safety as a public health issue has aroused worldwide concern. The immunosensor exhibited good repeatability, reproducibility, stability, and selectivity in experiments with a possible interferent. This sensitivity is sufficient to detect AFB1 in food according to regulatory agencies. With EIS as the principle of detection, the immunosensor could detect AFB1 in the range from 1 to 30 ng The architecture of the immunosensor was confirmed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and electrochemical impedance spectroscopy (EIS), including the effective immobilization of the active layer of anti-AFB1. The working electrode was functionalized with a drop-cast film of multiwalled carbon nanotubes (MWCNT)/chitosan on which a layer of anti-AFB1 monoclonal antibodies was immobilized covalently. The immunosensor was prepared with a waterproof paper substrate and low-cost graphite-based conductive ink through a simple cut-printing method. We report a paper-based electrochemical immunosensor made with sustainable materials to detect aflatoxin B1 (AFB1), a highly toxic, carcinogenic mycotoxin found in food.
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